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Kinetic-life cell imaging unraveled significantly increased relative confluence of MSC-CM exposed EGFP-SKBR3.
Transduction efficiency was approximately 80%, as evaluated qualitatively by relative confluence determination (before and after puromycin selection).
While their movement from an area of relative confluence to the denuded area is fascinating in itself, it is likely a reasonably accurate reflection of their motility in vivo, e.g., in ongoing wound healing of necrosed muscle in post-MI heart and/or stroma.
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The initial cell population size was 10% relative to confluence.
Drug effect on cell proliferation was analyzed in real time over a period of 96 120 h using the xCELLigence cell analyzer (Roche, Basel, Switzerland) that measures electrical impedance imposed by adherent cells and is expressed as a value relative to confluence (cell index).
Wound width, wound confluence or relative wound density were automatically measured by the IncuCyte software.
Cell migration was measured by three integrated metrics: wound width, wound confluence and relative wound density, using the IncuCyte software.
The data were analyzed by three integrated metrics: wound width, wound confluence or relative wound density automatically measured by the IncuCyte software.
In addition, total cell lysate samples were collected from HT1080 cells at 48 and 72 hr after confluence and the relative levels of peroxiredoxin-2 were quantified by densitometry after normalizing to a β actin loading control (Fig. 5C).
HeLa cells were seeded at a density of about 20% confluence (designated as a relative starting cell number of 100%) and then incubated at these curcuminoid concentrations for three days.
"The world is fundamentally a set of relative risks," Mr. Grumet added, noting the confluence of disasters in coal mining, oil drilling and nuclear plant operations.
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