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Fig. 5 Relative calculation times and closure approximation.

2, the layout for the contact force measurement is introduced; Sect. 3 describes the FEM model and the relative calculation; in Sect.

Notice the qualitative difference and similarity between b-c and b-d, respectively (a) Relative calculation times (b) Evolution of (bar {boldsymbol {N}}) with discrete model (c) Evolution of (bar {boldsymbol {N}}) with quadratic closure (d) Evolution of (bar {boldsymbol {N}}) with IBOF closure.

For relative calculation the Delta Ct method was used [ABI PRISM® 7000 Sequence Detection System and Applied Biosystems 7500 Real-Time PCR System - User Bulletin, Applied Biosystems] once all assays met the amplification efficiency criteria of 100% ±10%[Application Note 127AP05-02].

This will influence the relative calculations.

Despite currently still being computationally expensive, at this level of accuracy it is easy to recognize the great potential for application in lead optimization campaigns in a near future, complementing relative calculations.

13617_2012_5_MOESM2_ESM.doc Additional file 2: Absolute and relative resolution calculation (DOC).

It shows that the analytical model has relative high calculation accuracy.

The results show that the analytical model has relative high calculation accuracy.

In addition, EASM model also has good stability and relative economic calculation CPU time-consuming.

For relative permeability calculation purposes, the immobile solid phases (hydrate and ice phases) were treated as parts of porous rock, and normalized aqueous and free gas saturations were used in relative permeability calculation (see Eq. 5).