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The relative binding activities of C/EBPβ and HIF1α were assessed by qPCR.
We obtained similar relative binding affinity increases and assay precisions.
The relative binding affinities can be simultaneously determined.
Wang, L. et al. Modeling local structural rearrangements using FEP/REST: application to relative binding affinity predictions of CDK2 inhibitors.
Solid-phase binding assays were performed to determine the relative binding of CCBE1-175 to VEGFR-3.
We calculated the relative binding free energy of Na+ ions at the Na1, Na2 and Na3 binding sites.
Among the three drugs that bound to the DNA-Topo I complex, SN-38 had the strongest relative binding affinity, and CPT had the weakest relative binding affinity.
The relative binding of B2M KO cells was similar between Pregsure© BVD-vaccinated BNP and non-BNP dams and ranged between 9 54% (Fig. 3d).
Then, relative binding free energies were calculated to find the most appropriate molecule structure.
Unlike previous approaches, this framework includes not only examples of known interactions but also quantitative information about the relative binding affinities between different protein-DNA configurations.
Relative binding affinities were tested by radioligand displacement assays versus PMP-BSA (pentamannosyl phosphate-bovine serum albumin).
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