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Based on results from an affinity-purified anti-G544 antibody, relative assay sensitivity was estimated to be 1690 ng/mL in 100% serum when a cut point factor of 2 was used, which improved to 80 ng/mL with a statistically derived cut point of 1.48.
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The relative electrolyte leakage (REL) assay was performed according to the protocol previously described [ 45].
Figure 6 presents the relative luciferase assay of pGEF1I and pGEF1Idel, and is a typical dataset from a dual-luciferase assay: here six independent infiltrations were assayed for each of the plasmids under investigation.
After 24 h, the cells were harvested for relative luciferase assay analysis.
The cell proliferation was assessed by both ELISA-based relative proliferation assay and by counting the cells in S phase (Propidium iodide staining for DNA cell cycle analysis).
A relative quantitative assay uses a response concentration calibration with reference standards that are not fully representative of the biomarker.
We have developed a fast, sensitive and specific relative quantification assay of HIV-1 proviral DNA in purified CD4+ cells.
Gene expression analyses of ACO, MCAD, FAT, and UCP-2 were performed by Relative RQ assay using 18S as an internal control.
The relative activity assay with pNPG as the substrate showed that the BGL activity in the supernatant of KR7 was not improved, being 2.5-fold lower than KR5.
A relative quantification assay of HIV-1 proviral DNA in purified CD4+ cells was developed on the LightCycler® real-time PCR instrument expressed per 106 cellscells as derived from estimated by the quantification of the β-globin gene.
To accomplish this end and to approximate a relevant dose, we first performed a relative toxicity assay to characterize the general response of the embryos to a range of Pb concentrations.
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