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We have compared the biochemical activity and biophysical properties of two closely related active mariner transposases, Mos1 and Mboumar-9, and tested the cross-reactivity of the enzymes on each other's inverted repeats.
However, structural information from the activated β2AR structure was not transferrable to the closely related active dopamine D2 receptor structure, suggesting that the agonist state is more particular to a given GPCR-ligand pair.
The Beaver Dam study related active smoking (or smoking history) with a higher prevalence of dry eye [23].
Compstatin and related active analogs showed little or no inhibition of clotting or key enzymes in the clotting cascade nor did they appear to have significant cytotoxicity.
Although the kinase catalytic domain is highly conserved, protein kinase crystal structures have revealed considerable structural differences between closely related active and highly specific inactive forms of kinases [12] [17].
Based on this analysis, we postulated that the closely related active site of mtFabH could be expected to form an equivalent H-bonding network with TLM, and any new isosteric scaffold would need to maintain many of these important interactions.
A number of closely related taxa, assumed to contain related active compounds could then be screened.
To test whether the active β2AR structure could template a closely related active GPCR structure, we modeled and virtually screened an active dopamine D2 receptor (DRD2) structure.
Both genes Ψ1 and Ψ2 are thus far unique and there is no evidence of closely related active genes as evidenced by matching ESTs.
Comparison with the closely related active isoform VRK2 showed that VRK3 stabilizes a pseudoactive conformation by mimicking an ATP bound state by acidic residues.
Sequence comparisons reveal that Mos1 and Mboumar-9 transposases are the most closely related active mariner transposases described to date, and they share 68% identical amino acid sequences.
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