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Furthermore, the more closely related the template set of proteins is to the target genome, the less likely our method is to be confounded by gene duplication events that have occurred after the template and target lineages have diverged.
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Standard curves relating the initial template copy number to the fluorescence and amplification cycle were generated using the amplified PCR product as a template, and were then used to calculate the DNA copy number in each sample.
Selected compounds are structurally related to the template or can be present in biological samples.
The formation of Bi2O3-a (a = 0.2-0.4) phase is related to the template effect of structurally ordered silica.
This work is more related to the template-database approach of motion capture animation, as it was described in Section 1, than to the approach we describe in the present article.
The alignments of nematode vs. reference proteins were applied as restraints of the target proteins related to the templates, with other parameters set to default.
This method addresses an important source of uncertainty for dPCR-based quantification related to whether the template DNA is in single-stranded or double-stranded form [ 38].
In this sense, the method can most closely be related to template analysis [ 31], in which an a priori template is used to code the data.
Fig. 6 a Unbound chain (PDB ID: 1bbs, CHAIN ID: A, blue) and related appropriate template (PDB ID: 1hrn, CHAIN ID: B, yellow).
b Unbound chain (PDB ID: 1bbs, CHAIN ID: A, blue) and related appropriate template (PDB ID: 3g6z, CHAIN ID: A, yellow).
A further alternative involved switching to a cyclic carbon-linked side chain attached to a pyrazolopyrimidine scaffold; this closely related bicyclic template was known to show activity against this target.
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