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The subsequent increase in Flt3/STAT3 signaling in turn upregulates PU.1, thereby reinforcing expression of the core DC genes.
They suggested that assembly and/or stability of nucleosomes that occupy ncRNA promoters plays a key role in restricting their expression and reinforcing expression of the adjacent mRNA gene.
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This result indicated that the rate-limiting at polymerization step remained even with the reinforced expression of PHA synthase.
Immunohistochemical studies have shown the reinforced expression of Stat proteins in rheumatoid synovial tissues, which suggests the importance of the IFN-γ/JAK/Stat pathway [ 28, 29].
We show that reinforced expression of Bim and Bid in HTLV-1-infected T-cell lines sensitizes CD95/TRAIL- and anticancer drug-induced apoptosis.
Indeed, we find that INPP1 knockdown in SKOV3 cells leads to a marked downregulation in glucose transporter 1 (GLUT4), GLUT4, and hexokinase (HK2) expression, and we show partial to full reversal of these changes upon reinforced expression of INPP1.
The specificity of these metabolite changes were confirmed by two independent siRNA oligonucleotides targeting INPP1 as well as partial to full rescue of metabolite changes by reinforced expression of INPP1 in siINPP1 SKOV3 cells (Supplementary Figure S2A,B).
We confirmed the specificity of the INPP1 knockdown effects by recapitulating our antimigratory effects with two independent siRNA oligonucleotides for INPP1 as well as partially to fully rescuing the migratory defect with reinforced expression of INPP1 in siINPP1 SKOV3 cells (Supplementary Figure S1E,H).
As suggested by Aberdam and Mantovani 7, we cannot exclude the possibility that our mixed background (C57Bl6/129, 50 50) did not reinforce expression and stability of the truncated isoforms.
The dazed, breeze-teased suffocation of a country weekend — the Sunday stuffy-sofa feeling — finds reinforced expression in the tight-knit stasis of the drawing, its crammed elegance.
This suggests that DNA hypomethylation was often a consequence of the initiation of expression rather than its precedent, although it might thereby reinforce expression.
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