Sentence examples for regulatory isolation from inspiring English sources

Flow cytometry of each fraction showed that the stringency of CD25+ cell selection by the Miltenyi CD4+CD25+ regulatory isolation kit and Miltenyi anti-CD25 beads was comparable.

Purified PBMC were enriched for CD4+ cells and then separated into CD4+CD25hi and CD4+CD25− fractions using magnetic beads (Miltenyi CD4+CD25+ regulatory isolation kit).

CD4+CD25− and CD4+CD25+ cell populations were isolated using Dynabeads T regulatory cell isolation kit (Invitrogen, UK) following manufacturers instructions.

Next, CD4+CD25+ T cells were separated from CD4+CD25neg T cells on the AutoMACS in two repetitive separation steps using CD4+CD25+ T Regulatory Cell Isolation Kit (Milltenyi Biotec).

In the cell-proliferation experiments (Fig. 1D and 1E), that do not require a very high level of purity, we used a magnetic bead-based procedure to isolate Tregs: the Regulatory T Cell Isolation kit (Miltenyi Biotec) or the combination of a human CD4 T cell enrichment cocktail and a human CD25 selection kit (StemCell Technologies, Vancouver, Canada).

Tregs and Teffs were isolated using mouse regulatory T-cell isolation kits (Miltenyi Biotec) following the manufacturer's instructions.

Human PBMCs from healthy volunteers were isolated by Ficoll centrifugation (GE Healthcare); Treg cells were isolated using 'human CD4+CD25+ Regulatory T cell Isolation Kit' (Miltenyi) according to manufacturer's instructions.

Cells were isolated using human CD4+CD25+ Regulatory T Cell Isolation Kit and AutoMACS device (both Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer's instructions.

CD4+CD25- T cells and CD14+ monocytes were isolated using a CD4+CD25+ Regulatory T Cell Isolation Kit (130-091-301; Miltenyi Biotec) and CD14 MicroBeads (130-050-201; Miltenyi Biotec) according to the manufacturer's instructions.

Sequential use of the CD4+CD25+ regulatory T cell isolation kit and the CD4+CD62L+ T cell isolation kit resulted in naïve FOXP3-negative T cells used for in vitro induction of FOXP3.

In some experiments, CD4+CD25+ T cells were isolated using a MACS CD4+CD25+ Regulatory T cell Isolation Kit (Miltenyi Biotech, Germany), performed according to manufacture's instructions.