In view of the hierarchical structure of bacterial transcriptional regulation, we constructed a hierarchical coexpression network at whole transcriptome level.
To confirm that the defects observed in lin-23 mutants were due to a lack of BAR-1/β-catenin regulation, we constructed double bar-1; lin-23 mutants.
To experimentally validate transcription of the non-exonic TARs and to describe their transcriptional regulation, we constructed a new array (designated the re-array) using five independent 36-mer probes to represent each of the 44,385 non-TE gene models and 25,313 TARs in the japonica genome (see Materials and Methods).
To test if any Hfq-dependent sRNAs contribute to the virulence regulation, we constructed deletion mutants of 15 small RNAs identified in this study (Additional file 1: Table S1).
In an attempt to gather additional data in support of direct regulation, we constructed a set of nuclear localized mCherry and GFP reporters using the promoters and 3′ UTR sequences of each target (see Methods).
Given the data above, and to explore whether small RNAs play a role in strain-specific and/or virulence gene regulation, we constructed a small RNA library from trophozoites of the E. histolytica Rahman strain.
Additionally, for the convenience of the input regulation, we construct an input signal generation device with the concentration of inducer I1 to control the input g1 of the genetic transistor circuit.
To test whether the destruction of the antisense complementarity alone (without structural change) abolishes regulation by Yfr1, we constructed another Yfr1 mutant.
In an effort to identify the human genes involved in the regulation of ERα, we constructed a high-throughput, cell-based, functional screening platform by linking a response element (ERE) with a reporter gene.
In order to investigate the role of MET4 in regulation of CYS3, we constructed the met4 mutant YPH500m4 and performed lacZ reporter assays using this strain (Table 2, the 3rd column).
