Parallel to the interviews a desk research focusing on literature review related to alternatives for complying with the SECA-regulations was conducted.
In this study, a comprehensive gene expression analysis at the levels of both transcriptional and post-transcriptional regulation was conducted to identify response markers using human genome array with TS-depleted human colon cancer HCT-C18 (TS-) cells and HCT-C18 (TS+) cells stably transfected with the human TS cDNA expression plasmid.
Based on luciferase reporter studies and EMSA, our data also demonstrated that SHOX2 directly regulates ISL1 expression and that this regulation is conducted via the binding to an intronic regulatory element of ISL1.
Regulation is conducted by inserting Fe, Ni, and diatomic ligands of HypA F proteins into the hydrogenase center for activation and maturation [ 53].
Gene-wise tests for differential regulation were conducted as paired tests based on the observation of different apoptosis rates between the experiments (see Additional file 1: Figure S3).
While most of the psychological studies on emotion regulation were conducted in laboratory setting (e.g. using emotion induction), the present study was applied in the educational field, capturing the situated, context-bound nature of emotion regulation (Hagenauer and Volet 2013).
As the presence of a consensus binding site is merely suggestive of a possible regulation, RUNX1 depletion was conducted by siRNA transfection.
A waiver of individual patient consent was provided in adherence to institutional regulations, and the study was conducted according to good clinical practice guidelines.
This study followed the ethical standards of responsible local committees and regulations from the participating countries, was conducted in accordance with the ethical principles of the Declaration of Helsinki, and is consistent with good clinical practice when applicable to a study of this nature.
For the further investigation into glucose regulation, multiple linear regression analysis was conducted.
The earliest attempt at imposing transcriptional regulation on constraint-based models was conducted by Palsson and coworkers who developed regulatory flux-balance analysis (rFBA) [ 21- 25] where, using Boolean logic, a transcriptional regulatory network was superimposed on an FBA model.
