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From these regulations, our study was classified as exempt from the informed consent requirement because this study was based on the collection of existing publicly available data, documents and records, and we recorded all data in a manner that subjects cannot be identified.
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Although it is not yet clear whether Fascin-1 is itself subject to miRNA regulation, our study has established post-transcriptional regulation of Fascin-1 by miR-145.
These regulations are classified as certain regulations in our study.
All information was made anonymous according to data protection regulations and our study was approved by the Behavioural Research Ethics Board at the Generalitat Valenciana.
A second miRNA identified in this study, miR-26a, exhibited a trend towards up-regulation in our study (>1.3 fold up-regulated in 5 out of 6 mice).
The miRNA hsa-miR-19a that also showed a high MI value and up-regulation in our study was reported to be up-regulated in lung cancer tissue [ 34, 35].
The miRNA hsa-miR-22 that showed a high MI value and up-regulation in our study, was recently also reported to be up-regulated in blood cells of lung cancer patients [ 10].
In addition to the Aux/IAA genes four members of the GH3 family that have also been shown to be induced by auxin exhibited a rapid and sustained 2- to 8-fold up-regulation in our study, again confirming the findings reported in a previous study on auxin regulation of gene expression using Affymetrix 8 k oligonucleotide arrays [ 8].
Furthermore, adult mice were used in the EAE experiment and for the regional expression study, arguing against a developmental restriction of EAAT2 expression regulation in our study.
Although, a separate study can be undertaken to explore RNF43-mediated p53 regulation but our study provides compelling evidences that RNF43 targets p53 for ubiquitination.
A PAL gene found under up-regulation in our study encodes a key enzyme for the biosynthesis of anthocyanin and other PCs.
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