However, predictably fine-tuning function (e.g., by adjusting ligand affinity, specificity, catalysis, and/or allosteric regulation) remains a challenge.
Despite a combination of modern technologies such as high density single nucleotide polymorphism (SNP) panels, transcriptional profiling, ChIP-on-chip data [5], [6], together with computational approaches including eQTL [7], eQED [8], Regulatory Potential [9] and Regulatory Impact Factors [10], decoding causal transcriptional regulation remains a challenge.
6 11 Therefore, glucose regulation remains a challenge in the ICU.
How to combine heterogeneous data sources for reliable prediction of transcriptional regulation is a challenge.
The understanding of the mechanisms of transcriptional regulation remains a challenge for molecular biologists in the post-genome era.
However, the diverse mechanisms of Hox regulation pose a challenge for elucidating the exact mechanisms by which Hox proteins determine cell identities and where in the molecular cascade they exert their effects.
Although small regulatory modules have been derived by combining gene expression data with computational and functional analysis of cis-regulatory elements (Basso et al., 2005; Boyer et al., 2005; Donaldson et al., 2005; Loh et al., 2006; Novershtern et al., 2011; Suzuki et al., 2009), understanding global transcriptional regulation remains a challenge.
The Verizon lawsuit was dismissed as premature, the court said, because federal regulations dictate that a challenge to new F.C.C. rules must come within 10 days after a new rule is published in the Federal Register.
To do this, we do not need more regulation, we need better regulation – a huge challenge, not least given the growing gap in earnings between regulators and the business leaders they police.
Any form of outside regulation would be a challenge to the long-standing practice of accounting firms' policing themselves.
