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Translational regulation was monitored using a reporter gene, and the effects of various combinations of native or mutated UTRs on RNA stability and translational regulation were characterized.
SF2/ASF down regulation was monitored by Western blotting using an SF2/ASF monoclonal antibody (AK96, Zymed Laboratories, San Fransicso, CA; Invitrogen).
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Thus, the term epigenetics is used in many studies, although, e.g., only transient changes of histone modifications or in gene regulation are monitored.
Since the present study uses the data of Zellmer et al. but focuses on the second phase of the experiment (24 hours after the exchange of culture medium), the aftereffects of the proliferation initiation and cell cycle regulation were monitored as well.
Compliance with working hour regulations was monitored and behavior modified where necessary.
To better characterise the long-lasting effects observed with single and double TGF-β1 pulse(s), transcription of selected fibrogenic genes relevant for the TGF-β1 signalling and collagen regulation pathway was monitored.
Even higher up-regulation of EED1 was monitored in tup1Δ mutant cells at 30°C (Fig. 7 B).
In another study by Chang et al. [ 49] VEGF up-regulation following PDT was monitored in the PC-3 prostate cancer model in immunodeficient mice.
The regulation of pOC-Luc was monitored by its luciferase activity following transfection into MG-63 cells.
In the same study, the expression of genes, selected from cellular stress response (apoptosis, metabolism, proteolysis, chaperone, redox regulation, and DNA repair) pathways, was monitored.
The up-regulation in affinity of αIIbβ3 was monitored by flow cytometry to measure the binding of FITC-labeled fibrinogen to the platelet surface.
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