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By comparing the results of beads array with that of LNA array, we see that the consistency of LNA array in predicting the regulation trends (up- or down-regulated) is better than beads array, consistent with the results of inter-platform reproducibility as assessed by correlation coefficients.
Those genes are mostly upregulated, however down regulation trends can also be observable (i.e. TGFBR1).
In each instance, the qRT-PCR confirmed regulation trends observed in the microarray analysis.
The inferred networks reflect the regulation trends over the whole set of 184 samples.
For these exemplary genes, it could be shown that protein expression visualized by immunohistochemistry followed the up- and down regulation trends of the microarray data (Supplementary Fig. 1).
For carcinomas, we observed the significant concordance of the regulation trends between the two studies, although the total RNA source and microarray platforms used for both experiments were not identical.
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Considering the rapid variation of waste composition and the more severe regulation trend of pollutant emission in this country, the importance of the development of a reliable computer program for a full-scale, stoker-type incinerator cannot be emphasized too much especially in the view of proper design and optimal determination of operating condition of existing and future constructed facility.
"I'm quite convinced the cannabis regulation trend will continue and gradually speed up in the course of the next decade, as it will be shown in practice that a legally regulated market can be introduced in a responsible manner.
"I'm quite convinced the cannabis regulation trend will continue and gradually speed up in the course of the next decade, as it will be shown in practice that a legally regulated market can be introduced in a responsible manner, consumption does not explode and multiple benefits become visible.
For all the other genes the RT-qPCR results confirmed the regulation trend of the microarrays, but without statistical significance (p-value > 0.05).
3) For genes with more than one probe, only the genes with all the probes showing the same regulation trend were kept for further analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com