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Although it is known that USA300 has altered regulation of selected global regulators and virulence genes compared with USA400, it is not known whether there are global differences in the transcription profiles between USA300 and USA400.
The activation of specific MAPK signal transduction pathways may provide a mechanism to regulate the expression of torpor-responsive genes or the regulation of selected downstream cellular processes.
Our gene set enrichment analysis suggested that NMD is essential for the proper regulation of selected transcript classes.
These results are somewhat surprising and suggest that the course of T cell activation is also driven by regulation of selected cellular metabolic pathways.
Due to microarray spot abnormalities, regulation of selected genes including the fsr locus was assessed by real-time quantitative RT-PCR (described in further detail below).
The inverse correlation observed between miR-18a and miR-19b levels with antithrombin mRNA, one potential target of these miRNAs, suggests that certain miRNAs may be involved in the regulation of selected hepatic haemostatic proteins during development by targeting mRNA coding for these proteins and be in part responsible of the observed decay in neonates [12].
Quantitative PCR analysis confirmed the differential regulation of selected genes by LPA and EGF.
The regulation of selected candidate genes was confirmed by real-time RT-PCR.
The regulation of selected genes was also addressed at the protein level.
Regulation of selected bean genes in response to bean rust infection was confirmed by qRT-PCR.
The regulation of selected genes was validated by real-time PCR.
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