NormFinder ranks reference genes according to the least estimated intra- and inter-group variation, which is more effective to control the influence of co-regulation of reference genes.
Several studies have shown that using a single housekeeping gene as a reference for renal studies may reflect regulation of the reference gene rather than the target gene [ 23, 24].
In order to inhibit the DC circulating current, there are some developed methods such as voltage and current double close-loop control, three-state hysteresis control, filter circuit elimination and regulation of the reference sine wave DC component (Chen et al. 2004; He and Xu 2012; He and Zhang 2008).
Moreover, several recent studies report that such variation may by due to active regulation of classical reference genes under certain experimental conditions (see: http://normalisation.gene-quantification.info).info
The second important point that should be stressed is the possible co-regulation of selected reference genes.
For instance, a recent report indicated the de-regulation of common reference genes in hepatocellular carcinoma (HCC) arising from hepatitis C virus (HCV) infected liver [ 12].
Indeed, we observed 1.8-fold up-regulation of a reference peptide of RanGAP1 in the TK6 nuclear extract, but not in the TK6 whole cell extract (data not shown).
Indeed, policy makers often frame the regulation of citizenship with reference to conceptions about the boundaries of the national community (Jensen, 2014).
This framework was applied in order to assess the developmental regulation of the two reference genes Actin11 and Elongation Factor 1-β in the apex of poplar root.
All genes that were assorted to signaling, RNA, DNA, and regulation of transcription were referenced in the group system regulation as these proteins play roles in regulatory processes in a cell.
Therefore, we examined the response of TA and its regulation with reference to IM application in these settings.
