The high CV error in this case, indicates that a lot of true regulation is missed.
In my view, essential regulatory components beyond VASP localization and regulation are missing here, but would certainly be required for a comprehensive view of why dorsal and ventral stress fibers display the discussed differences in vectorial actin polymerization.
In addition to these 10 genes or operons, another 24 involved in motility have a putative MrpC binding site located between -400 and +100 relative to their predicted TCS (see Additional file 7, column D; Additional file 6, description and process) but either developmental regulation was missed in the microarray experiments or MrpC does not regulate these genes.
We also noted that qCTF validated fewer than half of the hits from previous screens for genes that elevate CIN upon dosage reduction, whereas 40% (74/186) of the dcCIN hits, including many known to function in mitosis and cell cycle regulation, were missed in previous screens.
The BioQuali analysis of the network with respect to this set of variations resulted in an inconsistent diagnosis, indicating that regulations are missing over IGF1 to explain the new set of observations.
19 Regardless of the introduction of compulsory motorcycle helmet use producing a substantial increase in using of helmets among motorcyclists in China, 20 similar regulations were missing for bicyclists and E-bikers.
PMN may miss a true regulator if a full regulation path is missing (hurting recall), the reconstructed pathways are sensitive to noise in the interaction measurements, and certain types of interactions (protein modifications, chromatin occupancy, cellular localization), as well as alternative splicing, are not modeled in the simple interaction graph.
We believe that these two mechanisms work independently because we saw only a marginal delay in spindle formation either in the mts3.1 proteasome mutant, hrs1.T14A stabilized mutant, or in the hrs1.FA6 nonphosphorylatable mutant, presumably because, when one of either regulation (degradation or phosphorylation) is missing, the remaining regulation still turns off the Hrs1 function.
Our data suggest that there may be another level of E2F (and possibly Myc) transcriptional regulation that is missed by using reporters such as these.
