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Dephosphorylated TFEB shuttles to the nucleus and directly binds to a 10-base pair motif known as a CLEAR (coordinated lysosomal expression and regulation) element, which is enriched in the promoter of numerous autophagic and lysosomal genes (Sardiello et al., 2009; Settembre et al., 2011; Settembre et al., 2013b).
When an upstream promoter region (−2139 to −1172) was deleted, the promoter activity was found to elevate about 4-fold, indicating that a negative regulation element may exist in the region (Figure 2A).
This observation implies that the linc-sscg2561 may be a regulation element for Dnmt3a gene.
Therefore it was proposed that a regulation element controlled the levels of DNMT1 mRNA in cycling cells.
Almost 30percentt of private firms were involved in 'bioinformatics for DNA analysis; data mining' (for example, DNAStar) and over 20% conducted 'gene expression analysis; microarray analysis; or analysis of siRNAs and other regulation element' (for example, Ipsogen).
The best-known examples of RNA structures that are superimposed on protein coding regions come from viruses: e.g. the Rev response element of HIV1 [ 31] or the cis-acting regulation element (CRE) in picorna viruses [ 32].
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Both GRDBD and ERDBD belong to the zinc-finger family of DNA binding proteins and bind to GR regulation elements (GRE 32 and ER regulation elements (ERE 33, respectively.
Furthermore, some putative regulation elements of both miR-181 and miR-1 were located.
Deletion mutants, designed for a detailed evaluation of the methanol regulation elements (AOX2-UAS, AOX2-URS1, and AOX2-URS2) did not respond to the addition of oleic acid.
These elements or motifs, or splicing regulation elements (SREs), include well documented or predicted exon splicing enhancers and silencers (ESEs and ESSs), and intron splicing enhancers and silencers (ISEs and ISSs).
These deletions appear to disrupt regulation elements other than self-assembly that exist in MHC-IIB and are required for normal cytoskeletal association.
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