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The TF and sigma factor gene regulation datasets were from RegulonDB.
We exploited gene and protein properties, global genome regulation datasets, and conservation of interactions between budding and fission yeast to predict fission yeast protein interactions in silico.
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Analysis of mRNA-expression modifications induced by oxidative stress in WT cells revealed 1498 gene regulations (Dataset S1b).
We compare several widely used normalization approaches (VSN, LOWESS, quantile, T-quantile, Tukey's biweight scaling, Peng's method) applied to a selection of regulation microarray datasets, ranging from DNA methylation to transcription factor binding and histone modification studies.
Since previous studies on the HapMap samples have shown that complex traits [34] and pharmacologic phenotypes [15] [17] are affected by genetic variants that alter gene regulation, this dataset on miRNA-mediated gene regulation should be a tremendous resource to the scientific community.
There was an over-representation of genes involved in ECM and cytoskeleton formation and regulation in the datasets.
Those genes were then arranged according to their average fold regulation in all datasets (Table 1, 4-fold; Additional file 2: Table S2).
The resulting clusters are candidates for functional gene modules that share a common expression regulation across the datasets we have used.
Realizing that biological processes typically involve more complex mechanisms in vivo than in vitro, in the first part of this study we investigate the optimal conditions of ceRNA regulation in expression datasets derived from clinical samples.
Analysis of the gene expression patterns showed a total of 5926 transcripts undergoing significant up – or down regulation (GSE #12666, Dataset S1).
On the other hand, DKK3 is a tumor suppressor, showing strong down-regulation in the datasets from Chandran et al., Lapointe et al. and Varambally et al. (Fig. 4C).
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