A future aspect is to iteratively improve the initial regulation assumptions by e.g. using Bayesian statistics, coupled with an expectation-maximization algorithm.
For clusters that are active only under a small subset of conditions, there is less ground for a co-regulation assumption.
From the classical view of transcriptional regulation, this assumption means that we consider a TF to regulate a set of genes encoding a complex only if the TF physically interacts with at least one protein from that complex.
But the report was based, in part, on a predicted "low" likelihood of fast action on the F.D.A. regulation, on the assumption that Congress would be busy with other priorities.
Second, TFs are multifunctional and typically cooperate to activate or repress genes, exerting a more complicated effect on transcriptional regulation than the assumption of a simple linear sum of TF activity.
In summary, the application of Nascent-Seq and RNA-Seq to mammalian circadian gene expression regulation challenges two assumptions of the mammalian circadian field.
In the previous section we considered Ca2+-dependent Ca2+ channel regulation under the assumptions that Ca2+ channels have an intracellular Ca2+ binding site and Ca2+ ion binding closes the channels.
Third, both adaptive state feedback and adaptive measurement output feedback controllers are synthesized to solve the cooperative output regulation problem without the assumption that each follower knows the system matrix of the leader system.
We have developed the pipeline presented in Figure 2 to uncover additional layers of regulation based on the assumption that regulation proceeds differently upon different perturbations, therefore some regulatory events will only be detectable in subset of conditions.
In regulation studies however, this assumption does not hold since the samples comprising the two channels differ to a large extent.
Another important assumption in regulation microarrays is that a DNA-interacting protein is either bound or not bound (for ChIP) and that a target sequence is either methylated or not (for MeDIP).
