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"Symbolizing man's loss of control of the environment, water becomes a regulating vector replacing man's habitat following a hypothetical ice thaw," Ronsiaux continues.
splendens [ 25] have been efficient in regulating vector and pest mosquito populations, both under field and laboratory conditions [ 26].
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To determine if similar translation products were generated in living cells, the cDNAs were cloned in a tetracycline regulated vector and stably transfected into the Tet-ON human embryonic kidney cells (HEK293).
Recent developments in extrachromosomal vector technology have offered new ways of designing safer, physiologically regulated vectors for gene therapy.
Improved pharmacologically regulated vectors could be developed for clinical application.
The construction and in vitro testing of hypoxia regulated vectors was supported by the grant POIG 01.01.02-00-109/99 ' Innofative methodstem stem cell applications in medicine'.
However, because of the relapsing and remitting phases of disease, use of transcriptionally regulated vectors to achieve cytokine expression will be extremely important [ 18], adding another layer of safety to gene therapy.
Because p16 has been shown to suppress pRB expression in vitro, we hypothesised that pRB expression could provide a simple indicator for the functional integrity of the p16 molecule expressed from the Dox-regulated vector system.
EBNA-1 specific effects were in every case assessed by comparison with the appropriate EBNA-1 negative control and non-specific effects caused by doxycycline treatment were accounted for by excluding genes that were regulated in vector transfected BJAB-tTA.
In this paper, we describe a novel self-contained transcriptionally regulated plasmid vector encoding a TNF inhibitor which fulfills these requirements.
Regulated vectors are powerful tools in gene therapy but will require stringent long-term testing in chronic models of disease in order to demonstrate their full potential.
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