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Eukaryotes have subsequently taken advantage of their expanded genomes by selectively regulating sequences provided by TEs, as envisioned in many earlier scenarios.
The short macronuclear intergenic regions in Paramecium facilitate the search for putative regulating sequences.
As published data mention ZBTB2 recognition of p21 regulating sequences, we verified that this sequence specific binding was also alleviated by 5-hmC.
It addresses the presence of putative regulating sequences for gene expression.
The EST data aid in this classification, in the identification of putative regulating sequences such as TATA or CAAT boxes.
The modified pUC19 vector was constructed by generating NcoI and NotI sites at the terminal ends of the pUC19 wild-type TEM-1 gene sequence by site directed mutagenesis (QuickChange Mutagenesis) to allow the wild-type TEM-1 gene to be deleted (leaving the original upstream regulating sequences intact).
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This observation identifies a previously unrecognized role for hTERT Q169 in regulating sequence-specific interactions between the protein's N-terminus and ssDNA primers in vitro.
All sequences regulated by LPS/INFγ/APC with a calculated fold change of more than 2.0 (p<0.01) were subjected to one-way ANOVA factorial analysis, compared with the significantly regulated sequences (fold change >2, p value<0.01) by LPS/INFγ.
Functional annotation of consensus sequences was performed to define the differentially regulated sequences more precisely.
One can see that there are two major differences between p-value distributions in really regulated sequences and in the random sequence.
These files present data regarding best hits in GenBank, annotation of the differentially regulated sequences, E-values, Gene Ontology and other useful information (where available).
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