Sentence examples for regulated vector from inspiring English sources

To determine if similar translation products were generated in living cells, the cDNAs were cloned in a tetracycline regulated vector and stably transfected into the Tet-ON human embryonic kidney cells (HEK293).

These data illustrate that the regulated vector operates in vivo, that the tetracycline components of regulation are also non-immunogenic (given that they are expressed long-term), and that the tetracycline promoter P tet is not inactivated over the 71-day period of the experiment.

Recent studies on the integration patterns of different categories of retroviral vectors, the genotoxicity of long-terminal repeats (LTRs) and other genetic elements, the rise of lentiviral technology and the emergence of regulated vector systems providing tissue-restricted transgene expression and RNA interference, are profoundly changing the landscape of stem cell-based therapies.

This study illustrates the potential for performing gene therapy with a single injection of regulated vector in CIA.

In this improved regulated vector, we have incorporated an internal ribosome entry site (IRES) for co-expression of rtTA2S-M2 and tetR-KRAB which can result in decreased expression of the second gene [ 28].

Recent developments in extrachromosomal vector technology have offered new ways of designing safer, physiologically regulated vectors for gene therapy.

Improved pharmacologically regulated vectors could be developed for clinical application.

The construction and in vitro testing of hypoxia regulated vectors was supported by the grant POIG 01.01.02-00-109/99 ' Innofative methodstem stem cell applications in medicine'.

However, because of the relapsing and remitting phases of disease, use of transcriptionally regulated vectors to achieve cytokine expression will be extremely important [ 18], adding another layer of safety to gene therapy.

Regulated vectors are powerful tools in gene therapy but will require stringent long-term testing in chronic models of disease in order to demonstrate their full potential.

EBNA-1 specific effects were in every case assessed by comparison with the appropriate EBNA-1 negative control and non-specific effects caused by doxycycline treatment were accounted for by excluding genes that were regulated in vector transfected BJAB-tTA.