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We compared the regulated genes identified through SSH with the genes found to be differentially regulated using the GeneChip microarrays in a human dendritic cell (DC) differentiation paradigm.
The flow rate of the syringe pump was regulated using the PICO Espin 2.0 version software.
The mass transfer coefficient (k c ) can be regulated using the amount of equilibrium adsorption.
Results show that delivery rate can be remotely regulated using the frequency of magnetic stimulation.
Consequently, the feed rate was regulated using the single signal based on the fluorescence intensity of NADH.
Figure 9 schematically represents various prescriptions that illustrate how regeneration (R) can be regulated using the timing and intensity of volume reductions in the overstory.
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In all cases, microarray results were supported by those obtained by qRT-PCR: all genes that were identified as up-regulated using the AppChip2 also showed up-regulation following qRT-PCR analysis, and the same was true for selected down-regulated genes.
A similar situation was evident when Col-0 RNA was hybridized to both arrays, where 82% (422 out of 512) of the genes that were found significantly cold-regulated using the same-species arrays showed no significant regulation in cross-species array hybridizations.
Ontology analysis of genes that were transcriptionally up-regulated using the EASE algorithm [19] indicates that Myc-responsive nucleotide metabolic genes are highly statistically over-represented at p<10−7.
Subsequently, these probesets were not counted as down-regulated using the stringent criteria imposed in this study.
Compared to the 32 up-regulated genes, only 9 transcripts were down-regulated using the same criteria of p-value < 0.01 and estimated FC > 10.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com