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Several proteins displayed consistent up or down regulation as displayed by the peak seen in Figure 7 at 10180 m/z which is particularly striking as the normal group remains consistently low at all the time points whereas the diseased group displays an up regulated trend from an early time point.
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However, their levels displayed a down-regulated trend beyond simultaneous GSPE treatment.
Molecular data showed an up-regulated trend in kisspeptin mRNA and immunoreactivity levels of hypothalamic area arcuate but a down-regulation in GPR54 mRNA expression after P1 5 DBP treatment.
In our transcriptome data, both srbA and srbB showed an up-regulated trend during fermentation, but the expression level of srbA was constantly low, while srbB expressed in a high level from the beginning and increased dramatically during middle and late oxygen limitation phase, indicating SrbB has important roles in the transcriptional regulation in hypoxia fermentation of A. niger.
Our results indicate that not only was a trend conserved for the dose responsive effects, but also a time –responsive up and down-regulated trend among these compounds was conserved, implicating that a common underlying mechanism may be shared by these compounds.
Only Unigene0076273 encoding abscisic acid receptor showed a down-regulated trend.
Pattern B showed an up-regulated trend and included XIP-I and GPD.
Among those key node proteins, tsr and ptx1 showed a significantly down-regulated trend.
It displayed a generally up-regulated trend, especially in the last period.
Further gene set enrichment analysis (GSEA) on GO terms, pathway, and transcription factor targets suggested the main up-regulated trend to be in cell cycle and proliferation, and the main down-regulated trend to be metabolism.
In the brassinosteroid signaling pathway, 6 out of the 8 DEGs showed down-regulated trends, while 1 showed as an up-regulated trend (Table 5, Figure 4).
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