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Pfaffl, M. W., Tichopad, A., Prgomet, C. & Neuvians, T. P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper Excel-based tool using pair-wise correlations.
Interestingly, in the absence of ligand, TRs also can repress basal transcription of positively regulated target genes, suggesting that unliganded TR may have a distinct role in gene regulation.
Several transcription factor binding sites were enriched in these differentially regulated target genes, including CCAAT-displacement protein (CDP).
We conclude that, in MCF-7 cells, transcription of the estrogen receptor gene and of ERα regulated target genes is cell cycle regulated.
pfr and amiE (aliphatic amidase) riboprobes were also used as control apo-Fur and iron-bound Fur regulated target genes, respectively.
All HSV-1 amplicon vectors contain transactivator coding sequences and the regulated target genes; HET6C vectors also contain a tetracycline controlled transcriptional silencer tTS [35] (Figure 1A).
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We have developed a set of high-throughput screening (HTS -compatible assays capable of measuring regulated, target-specific posttranslational modifications in a mammalian cell-based format.
We also observed the reverse, in which down-regulated miRNAs led to up-regulated target genes.
p65 can physically associate with STAT3 and bind to TNF-α-regulated target promoters, such as MCP-1 and ICAM-1, to initiate gene transcription.
Changes in Hox-regulated target gene sets are, in general, likely to underlie the morphological divergence of homologous structures between animals.
Two impediments to understanding the role of Hox genes in morphological evolution have been the limited number of organisms in which Hox gene function can be studied and the paucity of known Hox-regulated target genes.
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