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These observations will allow further dissection of the effects of histone acetylation and the genetic pathways regulating stage conversion in this pathogenic parasite.
Recently, the ApiAP2 transcription factor family has been suggested to regulate stage-specific gene expression in the human parasite Plasmodium falciparum [3], [4].
Furthermore, Tcf7l1/Tcf3 regulates stage-specific WNT signaling during the reprogramming of fibroblasts into iPSCs [ 69], neural stem cell status [ 70], or epidermal progenitor status [ 71].
Gene expression in gametes is developmentally regulated and stage specific, and thus requires a precise and well-coordinated program of transcription regulation.
Our data shows that the soybean β-amylase gene in the cotyledon (see Table 2) is down regulated during stages 2, 3 and 4, and up regulated during stages 5, 6 and 7.
We found that the Kunitz trypsin inhibitor in the soybean cotyledon cell was steadily up regulated during stages 2, 3, 4 and 5 while down regulated during stages 6 and 7.
Grem1 regulated the stage-specific Wnt/β-catenin and BMP signaling activity on cardiomyogenesis.
Only 15% probe sets were up- or down regulated between stages (p<0.05).
Erythroid cell development is a highly regulated multi-stage process resulting in the production of red blood cells (RBCs).
Expression analysis revealed developmentally regulated and stage-specific expression patterns in all of the genes.
Figure 2 gives a comprehensive overview of the pathways found regulated between stages and diets.
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