Countering this idea, however, a number of studies suggest that repression of Gro targets is exquisitely sensitive to the level of Gro activity, and indeed one very important way to regulate repression seems to be the modulation of Gro activity levels through Gro phosphorylation [43], [44], [45].
To investigate if estrogen regulates repression of MT1 cluster in ERα + breast cancer cell types, we conducted 17β-estradiol (E2) and demethylating agent 5-Aza-2′-deoxycytidine (DAC) treatment in various breast cancer cell lines.
To examine whether the hypoxic repression of Dicer protein levels was regulated through repression of Dicer mRNA levels, we examined Dicer mRNA levels by RT PCR in MCF7 cells exposed to 0.1% O2 for 8, 16, 24 and 48 h, and observed significant down regulation of Dicer mRNA expression in hypoxia.
In animals, miRNA transfection experiments showed that target genes are regulated by repression.
Interestingly, rVvpE uniquely regulated Muc2 repression through two distinct proteins, PKC δ and ERK.
Here, we assessed the influence of the spatial distribution of the genes on the dynamics of 3-gene transcriptional ring networks regulated by repression, i.e. repressilator circuits, using individual-based modelling to simulate their dynamics in two and three space dimensions.
We next investigated the mechanism by which Brg1 regulates the repression of developmental regulators in Brg1-deleted mesoderm.
CBX3 has been reported to function in chromatin packaging and gene expression regulation and has also been found to possibly regulate euchromatin repression by associating with nucleosomes in heterochromatin [ 113].
This is broadly in agreement with the consensus in the literature, that Hif-1α is not thought to directly regulate transcriptional repression [ 75], but functional experiments to investigate the possibility direct Hif-1α mediated repression of gene expression would be of interest.
This shows that a strictly stoichiometric analysis using EMA can synthetically de-regulate catabolite repression (perhaps the most well-studied means of metabolic regulation).
