Sentence examples similar to regulated references for from inspiring English sources
rps19 and hprt genes were used as non-regulated references for the normalization of target gene expression [ 22, 23].
The optimal annealing time was 4 sec, whereas optimal annealing temperature was 72°C, the detailed description of the optimal time and temperature conditions for the PCR were describe in our previous paper [ 4]. rps19 and hprt genes were used as non-regulated references for the normalization of target gene expression.
rps19 and hprt genes were used as a non-regulated reference for the normalization of target gene expression (sequences and reaction conditions has been previously given an account of: [ 23- 25, 28, 29]. Quantitative RT-PCR was performed using fluorogenic SYBR Green and the Sequence Detection System, Fast 7500 (Applied Biosystems).
Rps19 and β-actin genes were used as the non-regulated reference genes for normalisation of target gene expression.
hprt and rps19 genes were used as non-regulated reference genes for normalization of target gene expression [ 22, 23].
The values for three non-regulated genes, Tb927.3.930, Tb927.8.7680 and Tb927.7.1830, were used as references for the relative quantification.
References for this article.
Supplementary references References for Supplementary Tables.
The primers designed for these genes and the statistical analysis (probability Pr(sample>reference) and Pr(sample < reference) for up- and down-regulated genes, respectively) of the data are shown in additional file 10: Table 8.
To access the statistical significance of expression ratios, we assumed a log-normal model and calculated the probability Pr(sample>reference) and Pr(sample < reference) for up- and down-regulated genes, respectively.
