Sentence examples for regulated reference from inspiring English sources
Furthermore, the ASO and MSO had no effect on the levels of β-Actin, the not regulated reference protein used in the above experiments (data not shown).
CaOvate expression was normalized against the non regulated reference gene pepper Actin [GenBank: AY572427].
Values were normalized against the copy numbers of the not regulated reference gene chloride intracellular channel 1 (CLIC1).
Rps19 and β-actin genes were used as the non-regulated reference genes for normalisation of target gene expression.
Consequently, adding additional co-regulated reference genes would not significantly improve the combined value of the genes as normalizers.
hprt and rps19 genes were used as non-regulated reference genes for normalization of target gene expression [ 22, 23].
To evaluate differences in gene expression a relative quantification method was chosen where the expression of the target gene is standardized by a non-regulated reference gene; consequently, three replicates of each sample and endogenous control were amplified.
To decrease the likelihood of identifying a group of optimal reference genes that are co-regulated, reference genes located on different chromosomes and involved in different basic cellular processes should be included.
rps19 and hprt genes were used as a non-regulated reference for the normalization of target gene expression (sequences and reaction conditions has been previously given an account of: [ 23- 25, 28, 29]. Quantitative RT-PCR was performed using fluorogenic SYBR Green and the Sequence Detection System, Fast 7500 (Applied Biosystems).
rps19 and hprt genes were used as non-regulated references for the normalization of target gene expression [ 22, 23].
The optimal annealing time was 4 sec, whereas optimal annealing temperature was 72°C, the detailed description of the optimal time and temperature conditions for the PCR were describe in our previous paper [ 4]. rps19 and hprt genes were used as non-regulated references for the normalization of target gene expression.
