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The fold change (FC) of gene expression ratios >1.3 and P <0.05 were set as significance criteria to identify genes whose expression was differentially regulated in the study group at four time points.
The schedule according to which outpatients were followed up was not regulated in the study protocol but left to the physicians in order to demonstrate real life patient care.
Only 22 of the genes found to be differentially expressed between cell line A and cell line B were also found to be regulated in the study of Ehrenkaufer and colleagues and only eleven of them are regulated in both studies in the same direction [ 5].
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The reason would be that, since we employed cerulenin during mutant screening step, we may have selected those individuals presenting mutations on genes present in the same metabolic pathways regulated in the studies above mentioned.
In this approach statistically significant JACSs correspond to heavy subnetworks in a similarity graph, with nodes inducing a connected subgraph in G C. The probabilistic model we propose also accommodates the use of gene-specific priors, reflecting our confidence that they are transcriptionally regulated in the studied conditions.
The poor numbers of HSPs identified as up- regulated in the oyster study and the continued expression of HSPs in our study might reflect the functional diversity of HSPs in molluscs.
A putative GATA factor is up regulated in the present study pointing towards inhibition/down regulation of Vg-transcription.
Calpain 3 was not abnormally regulated in the microarray study (data not shown).
Raf1 human (RAF proto-oncogene serine/threonine-protein kinase) was down regulated in the present study.
Accordingly, transcription of caspase-3 was not differentially regulated in the present study.
Genes for regulating various signaling pathways involved in developmental processes are up regulated in the present study.
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