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The remaining probe sets were then merged, and are in the manuscript reported as significantly regulated features representing one transcript cluster.
The high reproducibility of the results achieved with two different oligo sets is a good indication for their robustness, and underlines the importance of less stringent selection of regulated features, in order to avoid a large number of false negative results.
The effects of the mixture exposure rendered 103 DEGs, almost equally represented by up and down regulated features (55% and 45% respectively).
Deeper analysis of the specific pathways showed, pathways with multiple regulated features including sterol/steroid biosynthesis with 10 genes down-regulated in fish fed FO (Fig. 5).
This method has the advantage of being self-adjusting through the accurate estimation of the unregulated features distribution d0 and taking into account the d distribution of regulated features thus providing an option to impose power requirements.
This method has the advantage of being self-adjusting through the accurate estimation of the unregulated features distribution f d0) and taking into account the f(d|μ) distributions of regulated features, thus providing an option to impose power requirements.
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The significance level for differently regulated feature was set at a fold change of FC > 2 and a Benjamini-Hochberg correction false discovery rate of FDR < 0.05.
Importantly, the replication timing profile of the genome is a dynamic, developmentally regulated feature that is coordinated with the reprogramming of gene expression and repositioning of chromosome domains within the nucleus [ 45, 46, 51].
According to provisions of the Telecommunications Act of 1996, municipalities cannot prohibit cellular towers based on health concerns, although they are permitted to regulate features like height and location, and can also require that companies share the sites.
The wealth of identified miRNAs that regulate features of melanoma cell aggressiveness supports the use of isogenic cell lines with differential phenotype as a screening platform.
The fact that the majority of these markers have receptor, ion channel, calcium ion binding, and G-protein signaling activities (Fig. 4B) further suggests that HVC neurons tightly regulate features of cellular excitability, intracellular signaling, and cell-to-cell communication.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com