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Regulated expression of transgene production and function is of great importance for gene therapy.
In its initial form, it was designed for regulated expression in mammalian cells.
Here we describe vectors that allow regulated expression of Deinococcal genes for functional analysis.
The five rice WRKY transcription factors with binding sites identified in the OsEULS2 promoter all show stress regulated expression profiles.
Here we present data linking protein kinase C alpha (PKCα) to the regulated expression of multiple ECM components including SLRPs.
Regulated expression of GP on the cell surface allowed production of reporter viruses harboring different amounts of GP.
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Over-expression of OsNPF7.2 resulted in the up-regulated expression of OsIPTs, LONELY GUY (LOG), and CK-response regulators (OsARRs/OsRRs) (Fig. 5a).
For example, OsWRKY45, a negative regulator of BLS resistance (Tao et al. 2009), exhibited down-regulated expression in OsHsp18.0-CI OE but up-regulated expression in 9804-Rxo1.
Italics indicates up-regulated expression in YΔGP/XK/XI.
b ABA-regulated expression of OsPYLs was analyzed by quantitative RT-PCR.
Fig. 1 Tissue-specific and ABA-regulated expression of OsPYLs genes.
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