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Plants were grown from seeds in pots in a regulated chamber at 23°.
Plants were grown in pots in a regulated chamber at 23° under a 16:8-hr light/dark cycle.
Plants were grown from seeds in pots in a regulated chamber at 23° with a 16-hr light/8-hr dark cycle.
Oxygen consumption by C2C12 myoblasts was measured using a standard oxygen electrode (Strathkelvin Instruments, North Lanarkshire, Scotland) in a magnetically stirred, thermostatically regulated chamber at 30°C.
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At the time of the experiment, the flasks are removed from the growth incubator and placed inside the temperature-regulated chamber (37°C), on the vibration platform.
Animals were allowed to recover in a temperature-regulated chamber before returned to the home cage.
Coverslips were transferred to a temperature-regulated chamber (RC-21BRFS, Warner, Hamden, CT, USA) mounted on a Carl Zeiss Axio Observer.
Chondrocyte respiration was measured by using a Clark-style oxygen electrode (Instech, Plymouth Meeting, PA, USA) in a temperature-regulated chamber set to 37°C (Hansatech Instruments Ltd, Norfolk, UK).
During O2 consumption measurements, the cells were placed in thermostatically-regulated chambers (37°C) equipped with a magnetically stirred circulator.
CD spectra were recorded at 20°C using a Jasco J-715 spectropolarimeter (Jasco, Gross-Umstadt, Germany) equipped with a temperature-regulated sample chamber.
In order to ensure cell viability over long periods of time for adequate live cell imaging, a temperature-regulated environmental chamber was custom-built by In Vivo Scientific (St . Louis MO).
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