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A Rho GTPase switch can be regulated by three classes of regulators: GE Fs GAPs, and GDIs.
The protein stability of hypoxia-inducible factor-1 alpha (Hif-1α) and Hif-2α is regulated by three prolyl hydroxylase domain-containing protein isoforms (Phd1, Phd2 and Phd3).
Many functions of cells are regulated by three interactions, namely, with (1) extracellular matrices (ECMs), (2) neighboring cells, and (3) soluble biosignals such as growth factors.
V(D J recombination and transcription within the TCR alpha/delta locus are regulated by three characterized cis-acting elements: the TCR delta enhancer (Edelta), TCR alpha enhancer (Ealpha), and T early alpha (TEA) promoter.
ATF6, a protein of interest to us, was predicted to be regulated by three of the upregulated miRNAs - miR-145, miR-221 and miR-494 (Figure 1). Figure 1A depicts the full-length human ATF6 3′UTR with predicted binding locations for miR-145, miR-221 and miR-494, and Figure 1B shows the locations and base pair matches of their proposed binding sites, adapted from TargetScan 6.2.
These complexes are regulated by three major types of protein-protein interactions.
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Myc is regulated by two Ras effector pathways: the extracellular signal-regulated kinase (Erk) and phosphatidylinositol 3-kinase (PI3K) pathways.
The barrier properties are regulated by two components.
Then, 13% (33 of 243) of these genes were regulated by two TFs.
On average, each enhancer targeted three promoters and each promoter was regulated by two enhancers.
Genes located within the big circle located in the middle might be regulated by two or more TFs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com