Simulation results show that the proposed controller effectively regulate the response to origin in state space even under saturated controller input.
Hence, these results may suggest that Pol κ- immunodepleted extracts are not able to regulate the suppression of origin firing after UV-induced fork stalling and that Pol κ is epistatic to ATR/Chk1.
Quality control tools are different in terms of areas they cover the pellet-production cycle, but considering the raw materials, they regulate only the origin but not the components.
Furthermore, transcriptional activity regulates the replication origin activity [28] [30] and it seems possible that it might play a role in altering the replication fork progression [31] [33], even though it is not clear whether it enhances the fork rate due to already partly unwound DNA or impeding it because the DNA is blocked by proteins involved in transcription.
Nevertheless, histone acetylation is unlikely to be the sole chromatin modification to regulate the activation of replication origins and similar to the regulation of gene transcription may act in concert with a multitude of other chromatin modifications.
To explore how the chromosomal positioning and the consequent epigenetic environment can regulate the firing of replication origins, we relocated different ARS sequences to a common chromosomal position and determined their activation time in S phase.
Altogether, we propose that YAP is required in adult retinal stem cells to regulate the temporal firing of replication origins and quality control of replicated DNA.
Cells lacking the bias determinant replicated both the URA3 gene and ARS510 similar to control cells, suggesting that the bias determinant does not detectably regulate the activation time of flanking origins at its native location.
The closed-loop system can be proved to be globally stable in probability and the states can be regulated to the origin almost surely.
In addition, the equilibrium of interest is globally stable in probability and the outputs can be regulated to the origin almost surely when the drift and diffusion vector fields vanish at the origin.
