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Between 1218 and 1874 DEGs (12.8% to 19.8% of total DEGs) were specifically regulated at a single time point, including 600 to 1178 DEGs with up-regulation and 591 to 1038 DEGs with down-regulation.
To outline the specific PHO response in S. pombe for subsequent analysis, and to avoid indirect activation of non-phosphate starvation regulated genes, we performed a single time-dependent, genome-wide expression analysis of wild-type S. pombe cells in medium lacking inorganic phosphate (no-Pi conditions, see Additional file 1).
It hasn't been reviewed a single time.
Although it is technically about four time zones in width, China has a single time zone.
Not a single time.
one day, ever, not a single time.
This is a single time fee.
Is it a single-time frame story?
Just tax it, regulate it, give it a license.
"So we have a plan to regulate it".
It would be a huge mistake to heavily regulate it".
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