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This recursion can be adapted to efficiently generate only regular transcripts by keeping track of the current block indel and the last block indel or block match.
In addition we examined the nc-rRNA, which extends from about −1000 to at least +300.Its exact extent from the transcription start site downstream could not be determined because of overlap with the regular rRNA transcript.
We define an edit transcript as "regular" if the following three conditions on indels are satisfied.
In the LL condition (the regular culture condition), LHCF2 transcript levels were significantly down-regulated in the dark period and significantly up-regulated in the light period compared to the transcript level at the onset of darkness.
This method is especially strong for revealing similarities across transcripts; however, the regular review and re-review of transcripts also potentially highlights differences between them.
The list was refined through repeated reading of transcripts and regular discussions about ideas or issues arising.
The lead author reviewed all transcripts and regular team discussions ensured the themes identified were tested for coherence and validity.
SB and GT initially analysed a random selection of eight transcripts, with regular meetings to discuss any variability in the arising themes and to generate an agreed set of common themes across the interviews.
Apart from debriefing between the first and second authors during the data collection phase, team members independently reviewed the transcripts and regular team meetings were held during data analysis to explore patients' underlying reasoning, to discuss deviant cases and to reach agreement on recurrent themes based on the pattern and relationship between the categories.
Overlaps that are shorter than the lower threshold are treated as insertions, whereas overlaps that are longer than the upper threshold are reported as regular duplicated regions in the transcript.
Longer transcripts have more regular expression level.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com