For CFU measurements, 106 cells each of PP9 (Kanr), PAO1 (Genr) and PP9 (Kanr)+PAO1 (Genr) (106+106) were inoculated in Tris+BA medium and at regular intervals samples were plated on luria agar+kanamicin (30 µg.ml−1) and luria agar+gentamicin (25 µg.ml−1) plates for CFU measurements.
At regular intervals, samples were taken from these populations and stored at -80°C.
At regular intervals, samples were taken and fixed with 500 μl of Fixing Solution (4% paraformaldehyde, 3.4% sucrose).
As opposed to fixed sampling schemes, which transmit at regular intervals, the sampling policy we propose is adaptive to the road network and the importance of the links that the vehicle traverses.
Sampling was done at regular interval and the samples were centrifuged at 10,000 rpm for 10 min followed by being kept at -20°C.
Measurements of a quality characteristic in terms of samples are taken from the production process at regular interval and the sample means are plotted on this chart.
At regular intervals, the samples were taken out to analyze acid value, density, kinematic viscosity, and thermogravimetric profile to monitor the quality of biodiesel.
BSA controls were monitored at regular intervals between samples to evaluate instrument performance.
At regular intervals, slurry samples were removed, using an N2 degassed syringe, and analyzed for various geochemical parameters.
L-tryptophan and its metabolites (kynurenine, kynurenic acid) were identified by their characteristic retention times as determined by injections of standards which were run at regular intervals during sample analysis.
At regular interval approximately 5 mL of samples was collected and centrifuged to obtain the nanoparticles for the determination of MB degradation.
