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These skin equivalents (SEs) develop superior epidermal architecture with regular differentiation and ultrastructure.
These SEs developed a superior epidermal architecture with regular differentiation and ultrastructure, which occurred also faster than in SEs based on collagen-DEs.
Chemiluminescence measurements performed after the regular differentiation period showed that a frequency of myoblastsFLPe as low as 0.04% could still give rise to a luciferase signal above that measured in cultures containing exclusively myoblastsGS.Luc (i.e. background [grey bar]) (Fig. 6B).
Co-cultures consisting of myoblastsFLPe and myoblastsGS.Luc kept in regular differentiation medium or in differentiation medium supplemented with either SB 203580 (0.5, 0.5 and 2.5 µM) or vehicle only, revealed a clear drug-dependent reduction in myoblast fusion activity as determined by chemiluminescence measurements (Fig. 7A).
In severe cases, only small nubs of cerebellar tissue are present, in which regular differentiation into lobes or folia is absent.
However, the addition of fibroblasts into the collagen matrix - either proliferative or postmitotic cells at a density of at least 2x10/ml – caused a sustained keratinocyte growth accompanied by regular differentiation.
Similar(53)
Loss of Sox2 and the ensuing upregulation of miR145 could then interfere with regular oligodendrocyte differentiation by preventing timely induction of these pro-differentiation factors.
Keratinocyte culture studies had revealed a strict dependency of regular epidermal differentiation on dermal interactions only accomplishable in three-dimensional skin models.
PV is associated with regular WBC differentiation, whereas MF is frequently associated with a mild increase in immature WBC.
Similar to the association between the levels of antibodies to serogroup C meningococcal polysaccharide and the levels of circulating memory B cells in infants vaccinated with the MenC glycoconjugate vaccine [29], these data suggest the replenishment of the plasma cell pool on a regular basis through differentiation of CS specific memory B cells [31].
Extracellular matrix modulus plays an important role in regulating cell morphology, proliferation and differentiation during regular and diseased states.
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