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In brief, after spheroplasting and regrowth, cells were washed three times with lysis buffer (0.4 M Sorbitol/150 mM potassium acetate/2 mM magnesium acetate/20 mM Pipes/KOH, pH 6.8/1 mM phenylmethylsulfonyl fluoride/10 μg/ml leupeptin/1 μg/ml pepstatin A/10 mM benzamidine).
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The regrowth of cells escaping cisplatin-paclitaxel toxicity was time-dependent with a marked increase in culture density as measured on days 7, 14 or 21 following initial chemotherapy.
The high expression of Ki67 and Bcl-2 in combination with the non-functional p53 could have contributed to cell tumourigenicity, regrowth of tumour cells, and the formation of radiation-damaged multinucleated giant cells with improper chromosome segregation and cell division behaviours [41, 45, 46].
This 23-kDa bFGF isoform alone was able to restore chemo-resistance by promoting chemo-residual cell survival and regrowth in SUM159 cells expressing bFGF shRNA.
In stark contrast, after 30 min of regrowth, whilst control cells formed robust radial microtubule arrays, hMsd1 knockdown cells exhibited disorganised microtubules with no focussed structures.
In contrast, subsequent cultivation of the cores in an organotypic culture revealed that significant cell death only occurred after 48 hours in the treatment group, but regrowth of tumour cells was observed after 72 hours.
The Norton-Simon model suggests that increasing the dose density of chemotherapy will increase efficacy by minimizing the opportunity for regrowth of tumor cells between cycles of chemotherapy.
Primarily, this appears to be because of rapid regrowth from viable cells at the rim of the tumour mass.
This concept is termed 'dose density' [ 13]; regrowth of resistant cells during and between cycles of chemotherapy is theoretically a principal cause of treatment failure.
By reducing the time between administrations, the purpose is to prevent tumor cells regrowth and minimize the possibility of CHT resistance.
Vesicles progress to ulcers and then undergo healing due to regrowth of susceptible cells: we assume that regrowth occurs according to a growth rate, λ or d × (S0 − S) with growth limited by S0, the carrying capacity of the system.
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