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In addition, non-toxic agents such as acetic acid solution [ 10] and cresyl violet [ 11] can be used for contrast enhancing, which facilitates the identification and registration of cells both in reflectance and fluorescence modes.
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However, automated whole-cell image registration of moving cells by pixel-wise tracking is still in great need for the high-resolution and efficient quantification of molecular signals in space and time.
The registration of basal cell carcinoma has not been quality-secured, and these cancer cases are excluded from the present analysis.
The motion of intercalating cells relative to the local tissue was computed by successive temporal registration of local groups of cells.
Therefore, the registration of the halftone cell locations can be done by detecting the edge of all the outer grids.
Based on image registration of the single-cell movement, we have developed automated image analysis methods to track and quantify the FRET signals within user-defined subcellular regions.
This is mainly due to the absence of approved legal regulations on the registration of medical products consisting of cells.
These calculations also indicate that from the perspective of mechanics and implantation by insertion the diameter of the electrode can be further reduced if electrical properties of the electrode and its interface with cells make registration of neural signals possible.
In the United States, embryonic stem cell isolation is not permitted using federal funds; however, since August 2001, the Bush government accepted registration of 78 human embryonic stem cell lines on which research could be carried out using government finance.
Inoculation of cells induced the mobilization of their internal Ca2+ and the registration of a large spike.
For RFP registration, the cells were excited with 568 nm and the fluorescence of RFP emission was detected with a BP610/20 nm filter.
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