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Lucy [ 60] and Trimpoly [ 61] were used to remove low-quality regions (quality score <20) as well as to trim poly-A signals.
Raw sequences were trimmed and cleaned using the Phred software [ 74], which resulted in the identification and removal of poor quality regions (quality cut-off of 20).
In the present study, we used only high quality regions (quality value ≥ 25) of raw shotgun reads from three different sets of genomic DNAs, which we used for determining the draft genome sequence of C. intestinalis[ 1].
Firstly, around 10% of the initial reads cannot be mapped (due to error rate, repetitive regions, quality of reads, etc).. Next, the 3 previous criteria filter out a high proportion of mapped bases.
Consistent with data from countries and regions, quality issues came third with global key informants; however, here, a focus on substandard medicines was considered to be more critical than counterfeit medicines as these were seen as a symptom of other system failures outside of quality assurance.
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Moreover, we introduce a novel indicator of the overlapping region quality which is used to verify the estimated rigid transformation and to improve the alignment robustness.
All analyzed SNPs were located within the high-quality region (Quality Value ≥20), and occasional low-quality passes were re-sequenced.
Worryingly, in the non-EU/EEA countries of the WHO European Region, quality assured gonococcal AMR surveillance only exist in 13% (3/23) of the countries [ 2].
From the 5'-end sequences, vector-derived sequences and low-quality regions (Phred quality value < 30) were trimmed using a combination of the cross_match program http://www.phrap.org and the phred program [ 38].
The contigs thus generated were screened with in-house Perl scripts to check for the low-quality regions (Phred quality values ≤ 25), and they were manually inspected for sequencing errors by using the Consed program [ 60].
After this initial evaluation, sequence data shorter than 100 bases in length or with many low-quality regions (Phred quality score ≤ 14, and more than 50% of its total length) were omitted.
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