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After excluding ambiguous regions, alignment lengths were 1,510 sites (599 of which were variable) for the gamma-Proteobacteria analysis and 1,547 sites (429 variable) for the Blochmannia-focused analysis.
In contrast to introns or coding regions, alignment of ribosomal DNA, to be biologically sensible, should account for secondary structure information (i.e., mature ribosomal RNA folded to form stems and loops maintained both by paired RNA regions and ribosomal proteins).
In T. pretoriensis CBS2187, at least two intron regions (alignment positions 212−304, and 1257−1287 in Figure S2) were different from T. delbrueckii but identical with the two S. cerevisiae strains.
In the context of more realistic mutation processes (e.g., indels that are the result of small duplications, rather than random amino acids, and substitution models that yield the equilibrium distribution of disordered regions), alignment errors could still account for some of the patterns of evolution observed in disordered regions.
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The six regions' alignments were individually checked for polymorphism using the samtools pileup function [ 77].
In the eutherian SPRN 3'- UTRs, we annotated 11 conserved regions, alignments of which are available on request.
For analysis of both 5'- and 3'-terminal regions, alignments of the two sequences were linked with 5 nt forced to be unpaired by folding constraints.
Using position data based upon the distance in kilobase pairs to pseudoautosomal regions, alignments between the a1 and a2 chromosomes along the length of the nonrecombining regions were not nearer to pseudoautosomal regions than expected by chance alone.
You are speculating about sintheny region (alignment of genes) but you do not want to show the information in aligned form!
Open image in new window Fig. 6 Nucleotide sequences alignment: a ITS-1 region alignment of Contracaecum larvae (sequences were deposited to the GenBank database under the accession numbers (KX580602-KX580607). b ITS-2 region alignment of Contracaecum larvae (sequences were deposited to the GenBank database under accession numbers (KX580607- KX5806013).Samples are labeled according to host fish.
Based on the IGS1 rDNA region alignment of hybrid strains and the Z. rouxii CECT 11923 and CECT 10425, in this work, we developed a pair of Zygosaccharomyces hybrid-specific primers, HibZF/HibZR.
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