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Multiple (∼5) high-power (×200, 0.5-mm2) fields were examined in each histologic slide from multiple regions of tissue, according to the CERAD regional sampling scheme.
For the four homoeologous regions studied here, TEs did not contribute substantially to sub-genome variation, perhaps due to regional sampling bias, but of the seven TEs identified in overlap regions, all were sub-genome-specific, similar to other reports of differential accumulation [ 50].
The regional sampling design used makes this study the largest scale genetic assay of a widespread Neotropical avian taxon published to date.
We have presented a method to downscale information from regional sampling surveys by poststratification using a land cover map derived from satellite-based measures of reflectance values.
Two vertical profiles of 4He concentration in aquitard pore water, augmented with regional sampling of aquifers above and below the aquitard were used to estimate fluid flux at multiple locations over a large spatial area.
In addition to the regional sampling, detailed vertical isotopic profiling was performed in one location by means of groundwater and sediment samples collected through the whole sequence of aquifers and aquitards.
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However, as shown in Figure 1, the pyrosequencing of additional regional samples will reveal more of these region-specific polymorphisms.
23 The sample for the 2005 survey consisted of two subsamples: i) the follow-up sample and ii) the supplementary regional sample (Danish counties were consolidated into five regions by 2005).
Our analysis therefore uses a regional sample, collected between 2001 and 2005 in a region in the south of Sweden.
Each region represented a distinct exposed population rather than a regional sample of the larger exposed population.
The distribution of duration of drug use was analysed to assess the composition of the regional samples and the epidemiological situation of drug use in the regions.
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