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The expression of predicted genes in BPH15 region was confirmed using RT-PCR.

The hyperoxic responsiveness of this region was confirmed using constructs with one or two copies of this region placed in minimal promoter-luciferase reporters.

The specificity of the microdissected regions was confirmed using The Rat Brain in Stereotaxic Coordinates, Fourth Edition Atlas (G. Paxinos and C. Watson).

Integrating the co-localization procedure and selective genotyping, allelically imbalanced genes are distributed across seven rice chromosomes – 1, 2, 3, 6, 8, 9, and 12. Two regions were confirmed using both in silico co-localization procedure and selective genotyping and therefore are the strongest candidates for further study on drought response.

The single-copy IR boundary regions were confirmed using region-specific primer pairs.

The binding of FoxO6 to this region in vivo was confirmed using CHIP assay, in which we found that FoxO6 specifically targeted this region without binding to the proximal promoter region.

This deletion, as well as a small gain found in a nearby region in SH-SY5Y was confirmed using MLPA.

The region-specific miRNA expression was confirmed using miRNA array on sub-dissected human foetal brain material.

Direct interaction of each region with the SKIP domain was confirmed using in vitro-translated proteins and GST-SKIP (Fig. 4E).

The interaction between the DEXI promoter region and intron 19 of CLEC16A was confirmed using several different primer sets.

The interaction between the C-terminal region of δ-catenin and GLTP also was confirmed using co-immunoprecipitation assays (Fig. 8C).