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The cerebellum was considered as the region of reference for the LUT normalization.
The PET scans were assessed visually and judged positive in case of detection of focally increased uptake above the surrounding tissue or increased uptake as compared to mediastinal blood pool activity which was used as region of reference in terms of FDG accumulation.
For the analysis of microarray datasets pertaining to these two neurodegenerative disorders, we matched the brain region of reference data (used to build the predictor) and target data (control and patient gene expression profiles) (Figure 1).
‡‡Primer location based on intergenic region of reference strain L2/434 downstream of ompA sequence.
The first reason is that the localization of microglial activation and ensuing abnormal TSPO density is generally unknown so one cannot select an anatomical region of reference a-priori.
The infratemporal crest of the sphenoid bone was chosen to be the anatomical region of reference to access the superior LPM, since this is the origin of the upper LPM.
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Similarly, background regions of reference and distorted images are used to assess quality of the background region by computing.
However, careful inspection of the corresponding ITS regions of reference chloroplast genomes available at NCBI (http://www.ncbi.nlm.nih.gov/genome/) revealed that this requires at least one base pair mismatch.
This allowed mapping of reads to regions of reference sequence with higher divergence.
Additional file 1: Table S3 summarizes the number of reads aligned to translated regions of reference snake venom toxin sequences.
Two distinct regions of reference located at two pulp horns of one μCT model were identified to allow for consecutive measurements in μCT slices and in histological sections.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com