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A root diameter of at least 1 mm was required for efficient regeneration and transformation.
Considering the importance of this plant, it is the need to have a well developed regeneration and transformation protocol.
The developed technique is especially effective for obtaining transplastomic plants that have low regeneration and transformation ability.
The higher regeneration and transformation rates were obtained from the seedlings cultured for 25 days in darkness followed by 10 days under light.
In this study, regeneration and transformation conditions, including explant selection and surface sterilization, use of different Agrobacterium strains, and co-culture processing, are optimized.
The camp was launched to explore innovative mobility conceptual designs on emerging cities with young talents, to provide inspiration and reference for the urban regeneration and transformation towards future mobility with the "South District of Shenzhen High-tech Industry Park (SHIP)" as the background site.
Although shoot regeneration and transformation systems are well developed in rice callus, the regeneration frequency is commonly low and is cultivars-dependent (Huang et al., 2002; Khaleda and Al-Forkan 2006; Dabul et al., 2009).
Our model describes normal tissue regeneration, malignant transformation, cancer incidence including dormant/transient tumors, and tumor evolution.
There is no efficient regeneration and transformation protocol available for this species.
For Lepidium, studies on in vitro regeneration and transformation are very limited.
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