Industrial enzymatic reactions requiring 1,4-NAD(P H2 to perform redox transformations often require convoluted coupled enzyme regeneration systems to regenerate 1,4-NAD(P H2 from NAD(P) and recycle the cofactor for as many turnovers as possible.
Glucose dehydrogenases (GluDH) from Bacillus species offer several advantages over other NAD(P H regeneration systems including high stability, inexpensive substrate, thermodynamically favorable reaction and flexibility to regenerate both NADH and NADPH.
Scott Hollister, a professor at the University of Michigan, Ann Arbor, is a co-founder of Tissue Regeneration Systems, a company that is commercializing technology his group is developing for skeletal reconstruction in the face, spine and extremities.
Numerous cofactor regeneration systems have been developed and systematical summarized in recent reviews [67-69] [67-69]
Multiple regeneration systems can be employed to achieve minimum flowrate and costs.
The optimized model is applied for two systems, partitioning regeneration systems of the fixed outlet impurity concentration and partitioning regeneration systems of the fixed impurity load removal ratio (RR) for water and hydrogen networks.
