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MACE consequently allows for preparation of libraries with varying read lengths and provides additional information regarding transcripts that do not possess an according restriction site for anchoring.
Regarding transcripts upregulated after NF90 was silenced, only HGF mRNA (in WI-38 cells) and IGFBP4 mRNA (in IDH4 cells), two NF90 targets, showed twofold enrichment.
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These transcripts have a nearly 2000 fold difference regarding transcript abundance.
Domain analysis yielded predictions regarding transcript and protein functions that can be experimentally validated.
High sequence divergence may affect binding efficiencies and thus lead to erroneous results regarding transcript abundance.
The newly developed deep-sequencing technologies make it possible to acquire both quantitative and qualitative information regarding transcript biology.
The taxonomic distribution served as a reference point for investigating the relative contribution of the coral animal and the algal symbionts regarding transcript expression discussed below.
Regarding transcript abundance, the set of differentially regulated genes is notably different when using promastigotes from the sand fly foregut instead of axenic cultures.
Regarding transcript contigs of SRA reads, Macrobrachium rosenbergii is the only species that currently has transcript contigs derived from an SRA dataset (81,411 reads for 50 million base pairs; [ 6]).
Regarding transcript abundance, the data at gastrula stage confirm the pattern seen when scoring cellular expression – in that the response of NvAth-like and NvSoxB(2) is stronger than that of NvAshA.
To validate the observed alterations in developing endosperms, we have used qRT-PCR, which confirmed that the observations regarding transcript accumulation were accurate and consistent with the findings of other laboratories undertaking similar studies (for an overview see [ 22]).
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